restriction enzyme bbsi (New England Biolabs)
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New England Biolabs
restriction enzyme bbsi
Restriction Enzyme Bbsi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1914 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bbsi/BbsI/pmc13095346-38-12-20
Average 98 stars, based on 1914 article reviews
Restriction Enzyme Bbsi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1914 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bbsi/BbsI/pmc13095346-38-12-20
Average 98 stars, based on 1914 article reviews
restriction enzyme bbsi - by Bioz Stars,
2026-09
98/100 stars
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other:Article Title: Chromosomal instability promotes cell migration and invasion via EFEMP1 secretion into extracellular vesicles. Article Snippet: To create CRISPR knockout cell lines targeting the human STAT1 gene (details in Expanded View Table EV2), we utilized the pSpCas9(BB)-2A-Puro V2.0 (PX459) plasmid (Addgene plasmid 62988, Feng Zhang) (Ran et al, 2013). Article Title: Protocol for Using CRISPR-Cas9 to Generate a Monocyte Cell Line Harboring a Single-Nucleotide Polymorphism Article Snippet: Clone Assay:Article Title: A mutational scar-based genome-wide map of DNA double-strand break repair. Article Snippet: Plasmid pU6-(BbsI)_CBh-Cas9-T2A-mCherry was a gift from Ralf Kuehn (Addgene# 64324). .. SgRNA sequences were cloned into the pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W backbone, the pLentimulti-Guide backbone, or into the pU6-(BbsI)_CBh-Cas9-T2A-mCherry backbone using two custom complementary DNA oligos (IDT) consisting of the target sequence and BbsI overhangs, which were melted, annealed and ligated (Promega, Catalogue# M180B) into the relevant Sequencing:Article Title: A mutational scar-based genome-wide map of DNA double-strand break repair. Article Snippet: Plasmid pU6-(BbsI)_CBh-Cas9-T2A-mCherry was a gift from Ralf Kuehn (Addgene# 64324). .. SgRNA sequences were cloned into the pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W backbone, the pLentimulti-Guide backbone, or into the pU6-(BbsI)_CBh-Cas9-T2A-mCherry backbone using two custom complementary DNA oligos (IDT) consisting of the target sequence and BbsI overhangs, which were melted, annealed and ligated (Promega, Catalogue# M180B) into the relevant Plasmid Preparation:Article Title: A mutational scar-based genome-wide map of DNA double-strand break repair. Article Snippet: Plasmid pU6-(BbsI)_CBh-Cas9-T2A-mCherry was a gift from Ralf Kuehn (Addgene# 64324). .. SgRNA sequences were cloned into the pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W backbone, the pLentimulti-Guide backbone, or into the pU6-(BbsI)_CBh-Cas9-T2A-mCherry backbone using two custom complementary DNA oligos (IDT) consisting of the target sequence and BbsI overhangs, which were melted, annealed and ligated (Promega, Catalogue# M180B) into the relevant Article Title: Pool-packaged AAV libraries exhibit extensive length-dependent and homology-dependent chimerism. Article Snippet: .. Cloning complex libraries of doubly barcoded AAV constructs with various inserts To clone a set of doubly barcoded AAV constructs, we digested plasmid AiP11839 (Addgene, 163509) with PacI and Article Title: Salmonella effector SteE reprograms the macrophage regulatory network to drive specific hyperactivation of STAT3 target genes. Article Snippet: .. STAT1 and STAT3 gRNAs (Table S15) were designed using CHOPCHOP67 and inserted in px330-GFP plasmids using Synthesized:Article Title: Dual role of ZIC2 during neural induction: from priming transcription factor to enhancer activator Article Snippet: .. Complementary oligonucleotides containing Article Title: Dual role of ZIC2 during neural induction: from priming transcription factor to enhancer activator. Article Snippet: .. Complementary oligonucleotides containing Article Title: HDAC1 and SATB1 positively regulate immune responses in chicken macrophages Article Snippet: .. Corresponding single-guide oligos were synthesized, annealed, and ligated into Cloning:Article Title: Pool-packaged AAV libraries exhibit extensive length-dependent and homology-dependent chimerism. Article Snippet: .. Cloning complex libraries of doubly barcoded AAV constructs with various inserts To clone a set of doubly barcoded AAV constructs, we digested plasmid AiP11839 (Addgene, 163509) with PacI and Bioprocessing:Article Title: Pool-packaged AAV libraries exhibit extensive length-dependent and homology-dependent chimerism. Article Snippet: .. Cloning complex libraries of doubly barcoded AAV constructs with various inserts To clone a set of doubly barcoded AAV constructs, we digested plasmid AiP11839 (Addgene, 163509) with PacI and Construct:Article Title: Pool-packaged AAV libraries exhibit extensive length-dependent and homology-dependent chimerism. Article Snippet: .. Cloning complex libraries of doubly barcoded AAV constructs with various inserts To clone a set of doubly barcoded AAV constructs, we digested plasmid AiP11839 (Addgene, 163509) with PacI and Agarose Gel Electrophoresis:Article Title: Pool-packaged AAV libraries exhibit extensive length-dependent and homology-dependent chimerism. Article Snippet: .. Cloning complex libraries of doubly barcoded AAV constructs with various inserts To clone a set of doubly barcoded AAV constructs, we digested plasmid AiP11839 (Addgene, 163509) with PacI and Ligation:Article Title: Salmonella effector SteE reprograms the macrophage regulatory network to drive specific hyperactivation of STAT3 target genes. Article Snippet: .. STAT1 and STAT3 gRNAs (Table S15) were designed using CHOPCHOP67 and inserted in px330-GFP plasmids using |